Institute of BiophysicsInstitute of Biophysics



  1. Yamamoto M, Du Q, Song J, Wang H, Watanabe A, Tanaka Y, Kawaguchi Y, Inoue JI, Matsuda Z. Cell-cell and virus-cell fusion assay-based analyses of alanine insertion mutants in the distal α9 portion of the JRFL gp41 subunit from HIV-1. J Biol Chem 294(14): 5677-5687. doi: 10.1074/jbc.RA118.004579, 2019. (APR 2019)


  1. Liu D, Wang H, Yamamoto M, Song J, Zhang R, Du Q, Kawaguchi Y, Inoue JI, Matsuda Z. Six-helix bundle completion in the distal C-terminal heptad repeat region of gp41 is required for efficient human immunodeficiency virus type 1 infection. Retrovirology 15(1): 27. doi: 10.1186/s12977-018-0410-9, 2018 (APR 2018)


  1. Yamamoto M, Matsuyama S, Li X, Takeda M, Kawaguchi Y, Inoue J, Matsuda Z. Identification of nafamostat as a potent inhibitor of Middle East respiratory syndrome (MERS) corona virus S-mediated membrane fusion using the split protein-based cell-cell fusion assay. Antimicrob Agents Chemother, 2016. (DOI:10.1128/AAC.01043-16) (Aug 22, 2016)


  1. Nakane S, Iwamoto A, Matsuda Z. The V4 and V5 variable loops of HIV-1 envelope glycoprotein are tolerant to insertion of green fluorescent protein and are useful targets for labeling. J Biol Chem 290(24):15279-91, 2015. (JUN 12, 2015)
  2. Nakane S, Matsuda Z. Dual split protein (DSP) assay to monitor cell-cell membrane fusion. Methods Mol Biol 1313:229-36, 2015. (doi: 10.1007/978-1-4939-2703-6_17.) (JUN 2015)
  3. Saw WT, Matsuda Z, Eisenberg RJ, Cohen GH, Atanasiu D. Using a split luciferase assay (SLA) to measure the kinetics of cell-cell fusion mediated by herpes simplex virus glycoproteins. Methods pii:S1046-2023(15)00227-3, 2015. (doi:10.1016/j.ymeth.2015.05.021) (MAY 26, 2015)
  4. Matsuda Z. Recent advance in the structural analysis of HIV-1 envelope protein. Sci China Life Sci May;58(5):420-424, 2015.(MAY 2015)


  1. Wang H, Li X, Nakane S, Liu S, Ishikawa H, Iwamoto A, Matsuda Z. Co-expression of foreign proteins tethered to HIV-1 envelope glycoprotein on the cell surface by introducing an intervening second membrane-spanning domain. PLoS ONE 9(5):e96790, 2014.


  1. Atanasiu D, Saw WT, Gallagher JR, Hannah BP, Matsuda Z, Whitbeck JC, Cohen GH, Eisenberg RJ. A dual split protein-based fusion assay reveals that mutations to HSV glycoprotein gB alter the kinetics of cell-cell fusion induced by HSV entry glycoproteins. J Virol, 87, 11332-11345, NOV 2013


  1. Ishikawa H, Meng F, Kondo N, Iwamoto A and Matsuda Z. Generation of a dual-functional split-reporter protein for monitoring membrane fusion using self-associating split GFP. Protein Engineering, Design & Selection (2012) doi: 10.1093/protein/gzs051, first published online: August 30, 2012.
  2. Toda T, Kuwahara K, Kondo N, Matsuda Z, Maeda Y, Maeda K, Sakaguchi N. Dynamic appearance of antigenic epitopes effective for viral neutralization during membrane fusion initiated by interactions between HIV-1 envelope proteins and CD4/CXCR4. Immunobiology 217(9): 864-872, 2012.


  1. Long Y, Meng F, Kondo N, Iwamoto A, Matsuda Z. Conserved arginine residue in the membrane-spanning domain of HIV-1 gp41 is required for efficient membrane fusion. Protein Cell. 2011;2(5):369-76.
  2. Kondo N, Miyauchi K, Matsuda Z. Monitoring viral-mediated membrane fusion using fluorescent reporter methods. Curr Protoc Cell Biol. 2011;Chapter 26:Unit 26.9.
  3. Yang W, Ding X, Deng J, Lu Y, Matsuda Z, Thiel A, Chen J, Deng H, Qin Z. Interferon-gamma negatively regulates Th17-mediated immunopathology during mouse hepatitis virus infection. J Mol Med. 2011;89(4):399-409.


  1. Liu S, Kondo N, Long Y, Xiao D, Iwamoto A, Matsuda Z. Membrane topology analysis of HIV-1 envelope glycoprotein gp41. Retrovirology. 2010;7:100.
  2. Miyauchi K, Curran AR, Long Y, Kondo N, Iwamoto A, Engelman DM, Matsuda Z. The membrane-spanning domain of gp41 plays a critical role in intracellular trafficking of the HIV envelope protein. Retrovirology. 2010;7:95
  3. Kondo N, Miyauchi K, Meng F, Iwamoto A, Matsuda Z. Conformational changes of the HIV-1 envelope protein during membrane fusion are inhibited by the replacement of its membrane-spanning domain. J Biol Chem. 2010;285(19):14681-8.


  1. Wang J, Kondo N, Long Y, Iwamoto A, Matsuda Z. Monitoring of HIV-1 envelope-mediated membrane fusion using modified split green fluorescent proteins. J Virol Methods. 2009;161(2):216-22.
  2. Kondo N, Ebihara A, Ru H, Kuramitsu S, Iwamoto A, Rao Z, Matsuda Z. Thermus thermophilus-derived protein tags that aid in preparation of insoluble viral proteins. Anal Biochem. 2009;385(2):278-85.


  1. Shimizu S, Urano E, Futahashi Y, Miyauchi K, Isogai M, Matsuda Z, Nohtomi K, Onogi T, Takebe Y, Yamamoto N, Komano J. Inhibiting lentiviral replication by HEXIM1, a cellular negative regulator of the CDK9/cyclin T complex. AIDS. 2007;21(5):575-82.
  2. Futahashi Y, Komano J, Urano E, Aoki T, Hamatake M, Miyauchi K, Yoshida T, Koyanagi Y, Matsuda Z, Yamamoto N. Separate elements are required for ligand-dependent and -independent internalization of metastatic potentiator CXCR4. Cancer Sci. 2007;98(3):373-9.
  3. Shimizu S, Urano E, Futahashi Y, Miyauchi K, Isogai M, Matsuda Z, Nohtomi K, Onogi T, Takebe Y, Yamamoto N, Komano J. Inhibiting lentiviral replication by HEXIM1, a cellular negative regulator of the CDK9/cyclin T complex. AIDS. 2007;21(5):575-82.
  4. Chiba-Mizutani T, Miura H, Matsuda M, Matsuda Z, Yokomaku Y, Miyauchi K, Nishizawa M, Yamamoto N, Sugiura W. New T-Cell-Based Lines with Two Luciferases for Accurately Evaluating Susceptibility to HIV-1 Drugs. J Clin Microbiol. 2007;45(2):477-87.


  1. Miyauchi K, Komano J, Myint L, Futahashi Y, Urano E, Matsuda Z, Chiba T, Miura H, Sugiura W, Yamamoto N. Rapid propagation of low-fitness drug-resistant mutants of human immunodeficiency virus type 1 by a streptococcal metabolite sparsomycin. Antivir Chem Chemother. 2006;17(4):167-74.
  2. Miyauchi K, Curran R, Matthews E, Komano J, Hoshino T, Engelman DM, Matsuda Z Mutations of Conserved Glycine Residues within the Membrane-Spanning Domain of Human Immunodeficiency Virus type 1 gp41 Can Inhibit Membrane Fusion and Incorporation of Env onto Virions. Jpn J Infect Dis. 2006;59,77-84.


  1. Miyauchi K, Komano J, Yokomaku Y, Sugiura W, Yamamoto N and Matsuda Z. Role of the specific amino acid sequence of the membrane-spanning domain of human immunodeficiency virus type 1 in membrane fusion J. Virol. 2005;79, 4720-29.